Danijela A Kostić,
Jasmina M Velicković 
,
Snezana S Mitić,
Milan N Mitić,
Sasa S Randelović
For correspondence:- Jasmina Velicković Email: jasmina@frad.rs Tel:+38118804722
Received: 18 May 2011 Accepted: 21 December 2011 Published: 21 February 2012
Citation: Kostić DA, Velicković JM, Mitić SS, Mitić MN, Randelović SS. Phenolic Content, and Antioxidant and Antimicrobial Activities of Crataegus Oxyacantha L (Rosaceae) Fruit Extract from Southeast Serbia. Trop J Pharm Res 2012; 11(1):117-124 doi: 10.4314/tjpr.v11i1.15
© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: The aim of this work was to determine the content of total phenols, total flavonoids, anthocyanins, as well as antioxidant and antimicrobial activities of hawthorn (Crataegus oxyacantha L.) alcohol, hydroalcohol and aqueous extracts.
Methods: The content of total phenols, flavonoids and anthocyanins of the alcohol, hydroalcohol and aqueous extracts of hawthorn were determined using spectrophotometric methods. Antioxidant assay was based on the measurement of DPPH absorbance at 517 nm caused by the reaction of DPPH with the test sample. Antimicrobial activity was evaluated by measuring the zone of inhibition against selected test microorganisms: Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Salmonella abony while antifungal activity was tested against two organisms: Aspergillus niger and Candida albicans.
Results: The results of spectrophotometric investigations indicate that the content of total phenol compounds in the investigated extracts varied from 2.12 to 30.63 mg GAE g-1 of fresh hawthorn sample. The content of anthocyanins ranged from 0.3207 to 3.168 mg of cyanidin-3-O-glucoside g-1 of fresh hawthorn fruit. The fruit extracts showed high antioxidant activity with DPPH radical transformation value as high as 89.9 % in the methanol-water (50/50, v/v%)) extract. The ethanol extract exhibited antimicrobial activity against all test microorganisms except two, Bacillus subtilis and Staphylococcus aureus, and one species of fungi, Aspergillus niger. Flavonoid structure influenced the extract’s selectivity towards Gram-positive and Gram negative bacteria.
Conclusion: Extracts of the fruit of Crataegus oxyacantha L. can be used as natural antioxidant and antimicrobial preparations.
Archives
News Updates
Fulltext in PDF